Raychelle
Burks
rmburks@hotmail.com
|
Currently,
I am working on a forensic science project in
partnership with Nebraska State Patrol Crime Lab
developing an explosives detection method based on
immunoextraction and capillary electrophoresis. |
Efthimia
Papstavros
papastav@unlserve.unl.edu
|
If
polymerization occurs in the presence of a
molecule of interest, the resulting polymer will
have an imprint of this molecule. When the imprint molecule is removed, a
binding site is created and the polymer will be
able to selectively rebind the molecule. Such polymers are known as molecularly
imprinted polymers (MIPs) and have been used in
affinity chromatography. I have been working on using them in
environmental separations, namely in studying
veterinary antibiotics in water. |
John
Schiel
schielj@bigred.unl.edu
|
One
focus of the current grant cycle is the
development of immunoassays for new target
molecules. Recently we have developed a number of
methods used to measure the free fraction of a
drug or hormone using microaffinity columns. Two examples of these are the one-site
immunometric assay and the displacement
immunoassay. In order to offer another viable
alternative for free fraction measurements, we
will also be testing a new method called: “Reverse Displacement Immunoassay.” Development of such an assay will require
the determination of kinetic rate constants to
understand the time domain necessary for free
fraction analysis. Chromatographic peak profiling, peak decay,
and band broadening studies will be used to
determine these constants. A new method of detection using near-IR
fluorescent dyes will also be tested. This will allow detection of physiological
concentrations (nM to pM) of free drug or hormone
without post column reactions. |
Chad
Briscoe
cbriscoe@yahoo.com
|
My
work focuses on the use of high performance
affinity chromatography with electrospray mass
spectrometry detection. This work will allow the
simultaneous determination of protein binding
constants in the presence of multiple analytes or
metabolites under physiological conditions. |
Krina
Joseph
ksjoseph@bigred.unl.edu
|
I am currently involved in two separate projects. The first project is looking at suitable replacement compounds for warfarin in drug-protein binding studies in order to reduce time and cost. The second project is characterizing the binding of sulfonylureas, drugs used in the treatment of type II diabetes, to HSA with increased glycation of the protein. This information is useful when determining adequate dosage for people with differing stages of diabetes. |
Omar
Barnaby
obarnaby@bigred.unl.edu
|
My research employs the use of MALDI-TOF mass spectrometry to assay the extent of glycation that occurs on Human Serum Albumin. I am particularly interested in quantifying the extent of glycation that occurs on the individual lysine residues. To do this an 18O labeling technique is employed, where a reference sample and a glycated sample is digested in 16O and 18O water respectively. The samples are then mixed and the 18O/16O ratios are determined. |
Zenghan Tong
zhtong@bigred.unl.edu
|
My research is focused on the use of ultrafast immunoextraction/ displacement assay for determining free drug fractions. Another project I will be working on is the study of factors that affect the performance of displacement assays and model displacement assays for drugs and their metabolites. |
Abby Fenner
afenner1@bigred.unl.edu
|
Most research dealing with drug-protein interaction is preformed using protein that is immobilized onto a support. This immobilization may alter the protein's binding ability. Currently, I am working on a new method of entrapping protein inside a silica pore. This research will determine whether an entrapped protein binds to drugs differently than an immobilized protein. |
Michelle Yoo
myoo@bigred.unl.edu
|
My research focuses on drug-protein interactions using HSA as the protein of interest. There are few projects I will be working on. One is researching imipramine and HSA and testosterone and protein interactions. I hope to be working on silica monoliths as well in the future. |
Yoon Jeong Jang
yjang2@bigred.unl.edu
|
My research focus is on studying drug-protein interactions of diazepam with human serum albumin. I'll also be working on attaching dyes to various drugs and studying their interactions with proteins. |
Sara Basiaga
sbasiage@unlserve.unl.edu
|
Type 2 diabetics have higher levels of fatty acids than non-diabetics. This affects a drug's ability to bind to HSA. I am studying how these fatty acids alter the ability of type 2 diabetes drugs to bind in
physiological conditions. |
